The NeoTYPE® DNA & RNA – Brain Profile uses targeted next‑generation sequencing (NGS) to detect single nucleotide variants, insertions/deletions, and gene fusions across 83 unique genes (62 DNA and 28 RNA; overlapping targets included). The profile also assesses microsatellite instability (MSI) and tumor mutational burden (TMB).
NGS – SNVs / Indels (62 genes + MSI and TMB)
Reflex rule: A microsatellite instability (MSI) NGS result of “indeterminate” will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available.
RNA Fusions (28 genes)
FISH – CNVs (9)
1p/19q co‑deletion, +7/‑10 (trisomy 7, monosomy 10), CDKN2A (p16) deletion, EGFR amplification, MET amplification, MYCN amplification, PDGFRA amplification, PTEN deletion
(FISH is global only)
IHC (1 biomarker)
PD‑L1 LDT (tech‑only available)
Test customization:
MGMT Promoter Methylation Analysis by PCR
Legacy Test information:
As of May 12, 2025, this test serves as replacement for the following test(s) that are no longer offered:
* Brain NGS Fusion Panel
NGS – SNVs / Indels (62 genes + MSI and TMB)
Reflex rule: A microsatellite instability (MSI) NGS result of “indeterminate” will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available.
RNA Fusions (28 genes)
FISH – CNVs (9)
1p/19q co‑deletion, +7/‑10 (trisomy 7, monosomy 10), CDKN2A (p16) deletion, EGFR amplification, MET amplification, MYCN amplification, PDGFRA amplification, PTEN deletion
(FISH is global only)
IHC (1 biomarker)
PD‑L1 LDT (tech‑only available)
Test customization:
MGMT Promoter Methylation Analysis by PCR
Legacy Test information:
As of May 12, 2025, this test serves as replacement for the following test(s) that are no longer offered:
* Brain NGS Fusion Panel