This test uses IgH/MAFB t(14;20) probes to detect gene rearrangements associated with myeloma and monoclonal gammopathy of undetermined significance (MGUS). This probe combination may be ordered separately or added to myeloma FISH panels.
IgH/MAFB t(14;20)
- FISH
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 1 slide, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
Storage and Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of collection.
CPT Code(s)*
Automated Analysis: 88374x1.
Manual Analysis: Codes may differ.
Turnaround time
3-5 Days
Level of Service
- Global
- Tech Only
Integrations
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New York Approved: Yes
Clinical Significance
The translocation (14;20) is rare in multiple myeloma (MM) but associated with a poor prognosis for that diagnosis. In contrast, t(14;20) in patients with MGUS is associated with a long-term stable disease.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
Fluorescence In Situ Hybridization (FISH)
| Translocation(s) | |||||
|---|---|---|---|---|---|
| MAFB/IgH t(14;20) | |||||
Last Updated: October 17, 2025