This FISH panel was designed to detect genetic abnormalities associated with Philadelphia chromosome-like (Ph-like) acute lymphoblastic leukemia (ALL) and B lymphoblastic leukemia/lymphoma (LBL). It includes the following probes:
PDGFRb (5q32)
BCR/ABL1-ASS1 t(9;22)
JAK2 (9p24.1)
EPOR (19p13.2)
CRLF2 (Xp22.33/Yp11.32)
Test Customization: Probes may be ordered separately.
STAT Processing (upon request): Available for BCR/ABL1/ASS1 t(9;22). Include "STAT" along with the requesting physician’s name and contact information for expedited results.
ALL FISH Panel (Ph-Like)
- FISH
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable.
Fresh, Unfixed Tissue: Tissue in RPMI.
Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: Minimum 5 slides, labeled with specimen type.
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of collection.
CPT Code(s)*
Automated Analysis: 88374x5.
Manual Analysis: 88377x5.
Turnaround time
3-5 Days
Level of Service
- Global
- Tech Only
Integrations
Request electronic integrations →
New York Approved: Yes
Clinical Significance
The ALL FISH Panel (Ph-like) detects cytogenetic abnormalities commonly associated with Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL). Patients with Ph-like B-ALL are associated with an unfavorable prognosis, but may potentially be amenable to inhibition with select tyrosine kinase inhibitors.
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
Fluorescence In Situ Hybridization (FISH)
| Translocation(s) | |||||
|---|---|---|---|---|---|
| BCR/ABL1/ASS1 t(9;22) | |||||
| Rearrangement(s) | |||||
|---|---|---|---|---|---|
| CRLF2 | EPOR | JAK2 | PDGFRB | ||
Last Updated: March 06, 2026